KMID : 1100720210410050489
|
|
Annals of Laboratory Medicine 2021 Volume.41 No. 5 p.489 ~ p.492
|
|
Genetic Characterization of Clostridium botulinum Isolated from the First Case of Infant Botulism in Korea
|
|
Jeon Jun-Ho
Choi Chi-Hwan Kim Jeong-Hyun Hyun Jung-Hee Choi Eun-Sun Choi Sang-Yoon Shin Yong-Woo Pyo Seong-Wook Kim Dae-Won Kang Byung-Hak Park Young-Joon Rhie Gi-Eun
|
|
Abstract
|
|
|
Botulism is a neuroparalytic disease caused by a neurotoxin produced by Clostridium botulinum. This study aimed to genetically characterize C. botulinum strain isolated from the first case of infant botulism in Korea reported on June 17, 2019. We isolated C. botulinum strain CB-27 from a stool sample of the patient and analyzed the toxin types and toxin gene cluster compositions of the strain using a mouse bioassay, real-time PCR, and genome sequencing. Toxin gene cluster analysis showed that strain CB-27 possesses a C. botulinum neurotoxin type A harboring an unexpressed B gene. Although the nucleotide and amino acid sequences of toxin genes as well as the toxin gene cluster arrangements in strain CB-27 were identical to those of the known strain CDC_69094, the total nucleotide sequences of the toxin gene clusters of CB-27 differed from those of CDC_69094 by 0.47%, indicating genetic diversity of toxin gene clusters of CB-27 among other previously reported C. botulinum strains. To our knowledge, this is the first description of a C. botulinum strain with two separate toxin gene clusters in Korea.
|
|
KEYWORD
|
|
Clostridium botulinum, Toxin type, Toxin gene cluster, Genetic diversity, Neurotoxin
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|